anti nrf2 (Proteintech)

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Proteintech anti nrf2
Anti Nrf2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 2093 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+nrf2+antibody/LC3+Antibody/pm42790822-87-25-27
Average 96 stars, based on 2093 article reviews

anti nrf2 - by Bioz Stars, 2026-10

96/100 stars

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Blocking Assay:

Article Title: Quercetin alleviates radiation-induced erectile dysfunction by modulating oxidative stress and apoptosis through the Nrf2/HO-1 pathway
Article Snippet: .. After fixation, cells were permeabilised with 0.3% Triton X-100 in PBS for 10 min at room temperature, followed by blocking with 5% BSA for one h. After blocking, cells were incubated overnight at 4 °C with primary antibodies diluted in BSA blocking buffer: anti-Nrf2 antibody (Proteintech, 80593-1-RR, 1:250) or anti-HO-1 antibody (Proteintech, 66743-1-Ig, 1:250). .. The following day, cells were washed three times with PBS (5 min each) and then incubated in the dark at room temperature for one h with the corresponding fluorescent secondary antibodies: Alexa Fluor 594 conjugated goat anti-rabbit IgG for Nrf2 (1:500, Proteintech) and Alexa Fluor 488 conjugated goat anti-mouse IgG for HO-1 (1:500, Proteintech).

Incubation:

Article Title: Targeting mitochondrial phosphatase PGAM5 alleviates ferroptosis and acute pancreatitis by upregulating NRF2-mediated FSP1 expression
Article Snippet: .. The 4 × 10 3 μg cell lysates were incubated overnight with 3 μg anti-NRF2 antibody (Proteintech, Cat16396-1-AP) or IgG antibody (Biodragon, Cat#BF02008) at 4 °C. .. The mix of cell lysates and antibody was then incubated overnight with 25 μL protein A/G magnetic beads (MCE, Cat#HY-K0202) at 4 °C.

Article Title: 7,8-Dihydroxyflavone protects acetaminophen induced liver injury through activating PI3K/Akt/NRF2/GPX4 mediated ferroptosis suppression.
Article Snippet: Acetaminophen (APAP)-induced liver injury remains a leading cause of acute liver failure, with limited therapeutic options beyond the narrow therapeutic window of N-acetylcysteine.. Emerging evidence highlights ferroptosis as a critical mediator of APAP hepatotoxicity.. Here, we investigated the hepatoprotective effects of 7,8dihydroxyflavone (7,8-DHF), a natural flavonoid with antioxidative properties, against APAP-induced liver injury and explored its underlying mechanisms.

Article Title: Ferulic acid activates Nrf2/HO-1 signaling axis to ameliorate neuronal Golgi stress by SKP2
Article Snippet: After the corresponding treatments, cells were harvested and lysed in ubiquitination lysis buffer (Thermo Fisher Scientific). .. Then, lysates (500 μg) were immunoprecipitated either with 2 μg of anti-Nrf2 antibody (Proteintech, 16396-1-AP, 1:6000) overnight at 4 ◦C, followed by 2 h incubation with magnetic Protein A/G beads. .. Following three wash cycles, ubiquitinated Nrf2 was detected by Western blot analysis using anti-ubiquitin conjugate-specific antibody (Abcam, ab140601, 1:1000).

Article Title: Targeting mitochondrial phosphatase PGAM5 alleviates ferroptosis and acute pancreatitis by upregulating NRF2-mediated FSP1 expression.
Article Snippet: .. The 4 × 103 μg cell lysates were incubated overnight with 3 μg anti-NRF2 antibody (proteintech, Cat16396-1-AP) or IgG antibody (Biodragon, Cat#BF02008) at 4°C. .. The mix of cell lysates and antibody then were incubated overnight with 25 μL protein A/G magnetic beads (MCE, Cat#HY-K0202) at 4°C.

Staining:

Fluorescence:

Microscopy:

Laser-Scanning Microscopy:

Article Title: β-Cryptoxanthin Confers Radioprotection against Intestinal Injury via NRF2-Mediated Antioxidant Response and Gut Microbiota Reprogramming
Article Snippet: Images were acquired using an EVOS inverted fluorescence microscope (Thermo Fisher, USA), and the number of γH2AX foci in randomly selected cells from each group were quantified. .. For NRF2 detection, anti-NRF2 antibody (Proteintech, 16396-1-AP) was used as the primary antibody, and images were acquired using a confocal laser scanning microscope (Leica, Germany). ..

Immunoprecipitation:

Binding Assay:

Article Title: IL-3 Modulates Microglia Polarization and Attenuates Neuroinflammation in Traumatic Brain Injury.
Article Snippet: The following parameters ere used in the sequence protocol: T2-weighted: TR: 2724 ms, E:33 ms, number of slices: 26, slice thickness = 0.8 mm, FOV: 35 m ×35 mm, Matrix = 256 × 256, scan time: 5 min 48s 698 ms, 2-weighted images in the sagittal plane were acquired using Bruker ParaVision 6.0 system (Bruker, Ettlingen, Germany). .. NRF2 binding proteins were 8 of 21 then captured with anti-NRF2 antibody (66504-1-Ig, Proteintech 1:200) or protein IgG beads. .. .16 HE Stains E staining was performed using an HE staining kit (Servicebio, uhan, China), according to the manufacturer’s instructions.

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